Cellprofiler measure fiber length manual#

Automatic quantification of muscle fiber cross-sectional area using the proposed method is a powerful tool that will increase sensitivity, objectivity, and efficiency in measuring muscle adaptation. Our automatic approach is accurate and represents a significant advancement in efficiency quantification of fiber area in muscle cross sections was reduced from 25–40 min/image to 15 s/image, while accommodating common quantification obstacles including morphological variation (e.g., heterogeneity in fiber size and fibrosis) and technical artifacts (e.g., processing defects and poor staining quality). The final muscle fiber boundaries are automatically delineated using a gradient vector flow deformable model. The proposed algorithm begins with ridge detection to enhance the muscle fiber boundaries, followed by robust seed detection based on concave area identification to find initial seeds for muscle fibers.

Cellprofiler measure fiber length manual#

The proposed automatic segmentation algorithm was evaluated against a semiautomatic method with manual annotation using 35 randomly selected cross-sectional muscle histochemical images. We have developed and validated an automatic image segmentation algorithm and compared it directly with commercially available semiautomatic software currently considered state of the art.

This process is labor intensive, time consuming, and prone to errors, leading to high interobserver variability. However, at this time, quantification of muscle fiber cross-sectional area is still a manual or, at best, a semiautomated process. Morphological characteristics of muscle fibers, such as fiber size, are critical factors that determine the health and function of the muscle.